J Korean Soc Microbiol.  1998 Oct;33(5):485-497.

Epidemiology of Serratia marcescens Isolates by Transferable Resistance Gene Analysis

• Seol SY
• Cho DT
• Lee YC
• Shin HS
• Chang HK
• Kim KS

Abstract

Conjugative R plasmids derived from 74 clinical isolates of Serratia marcescens were epidemiologically analyzed for antimicrobial resistance, EcoRI restriction endonuclease analysis and Southern hybridization with DHFR, TEM and SHV probe. 1. Resistance frequency of isolates against various B-lactam antibiotics was changed by year. 2. Twenty (27%) resistant strains transferred 32 R plasmids to E. coli or Klebsiella by mixed culture. Most strains isolated from 1994 to 1996 transferred only trimethoprim resistance but most strains isolated from 1997 did resistances against gentamicin (Gm) and B-lactams including ampicillin (Ap), carbenicillin (Cb), cefazolin (Cz), cefaloridine (Cl), cefamandole (Cn). 3. Ten plasmids of GmApCbCzC1Cn or GmApCbCzC1 pattern and 3 plasmids of TcSuGmTbApCbCzC1 pattern respectively showed identical EcoRI restriction endonuclease digestion patterns and hybridized fragment patterns with TEM-1 probe by Southern hybridization. These results indicate that the epidemic plasmids carrying blamM gene were present in this hospital in 1997 and molecular genetic analysis of R plasmids can be used to discriminate S. marcescens isolates for epidemiologic studies.

MeSH Terms

Ampicillin
Anti-Bacterial Agents
Carbenicillin
Cefamandole
Cefazolin
Cephaloridine
Digestion
DNA Restriction Enzymes
Epidemiologic Studies
Epidemiology*
Gentamicins
Klebsiella
Molecular Biology
Plasmids
R Factors
Serratia marcescens*
Serratia*
Trimethoprim Resistance
Ampicillin
Anti-Bacterial Agents
Carbenicillin
Cefamandole
Cefazolin
Cephaloridine
DNA Restriction Enzymes
Gentamicins