J Korean Soc Transplant.  1999 Dec;13(2):221-228.

Prostaglandin E1 Inhibits CD64 (Fc gamma RI) Expression on Monocyte in Cultured Peripheral Blood Mononuclear Cells

• Park JK
• Shin HJ
• Kim DK
• Shin JH
• Park DG
• Jun HJ
• Ryu JW
• Chung M
• Cho JT
• Kim JW
• Kim JT

Abstract

CD64 (Fc gamma RI) is the one of the three Fc gamma receptors on monocytes and represents a high affinity immunoglobin G receptor. CD64 is rapidly upregulated on monocyte in response to gamma interferone. X-linking of CD64 triggers an oxidative burst as well as antibody dependent cytotoxicity. In this experiments, peripheral blood mononuclear cells (PBMC) were separated and incubated with or without gamma interferone and PG E1. The samples were divided into four groups, the first was PBMC alone, the second was PBMC with gamma interferone 100 U/ml, the third was PBMC with gamma interferone 100 U/ml and Prostaglandin E1 1 micro M/L, and the fourth was PBMC with gamma interferone 100 U/ml and Prostaglandin E1 10 micro M /L. Flow cytometric measurements of CD64 on monocyte were performed at 0, 3, 6, and 9 hours after incubation and the mean fluorescence intensities (MFI) and the mean percentages of CD64(+) cell in monocytic gated area were obtained. After 6 hours of incubation, although there is no statistical significance, all gamma interferone added groups show the higher mean fluorescence intensity than PBMC alone group. Furthermore, at 6 and 9 hours of incubation, the mean percentages of CD64(+)cells between the PBMC with gamma interferone group and the PBMC with gamma interferone and PG E1 10 micro M/L group showed 74.83 +/- 9.72% vs. 34.07 +/-12.98%, 80.04 +/- 11.30% vs. 29.42 +/- 19.86% respectively, and there are statistical significances, p=0.05, p=0.05 respectively. It appears that PG E1 inhibits the expression of CD64 on monocyte.

Keyword

Monocyte; CD64; Prostaglandin E1

MeSH Terms

Alprostadil*
Fluorescence
Interferons
Monocytes*
Receptors, IgG
Respiratory Burst
Alprostadil
Interferons
Receptors, IgG