Korean J Anat.  2004 Oct;37(5):449-457.

Synthetic Bile Acid Derivative HS-1200-induced Apoptosis of Human Osteosarcoma Cells

• Kim GC
• Her YS
• Park JH
• Moon YS
• Yoo YH
• Shin SH
• Park BS

Abstract

Bile acids and synthetic its derivatives induced apoptosis in various kinds of cancer cells and had anticancer effects. However, it wasn`t discovered those materials have apoptosis induced effects on osteosarcoma cells. The present study was done to examine the synthetic bile acid derivatives induced apoptosis on osteosarcoma cells and such these apoptosis events. The synthetic bile acid derivatives, chenodeoxycholic acid (CDCA) induced the cell death on human osteosarcoma (HOS) cells contrary to ursodeoxycholic acid (UDCA). HS-1200, a synthetic derivative of CDCAs, was chosen to experiment apoptosis events in HOS cells. HOS cells treated with HS-1200 showed nucleus condensation, cytochrom c release, Bax/Bcl-xL alteration, activation of caspase-3 and caspase-activated deoxyribonuclease (CAD), and degradation of poly (ADP-ribose) polymerase (PARP). Though this study needs more investigations, these in vitro data suggest that treatment of the synthetic bile acid derivatives can give medical therapy on HOS cells.

Keyword

Synthetic bile acid; Apoptosis; Anticancer effect; Osteosarcoma cells

MeSH Terms

Apoptosis*
Bile Acids and Salts
Bile*
Caspase 3
Cell Death
Chenodeoxycholic Acid
Humans*
Osteosarcoma*
Ursodeoxycholic Acid
Bile Acids and Salts
Caspase 3
Chenodeoxycholic Acid
Ursodeoxycholic Acid