Korean J Anesthesiol.  2000 Jan;38(1):152-157. 10.4097/kjae.2000.38.1.152.

The Effect of Hypertonic Saline and Mannitol against Edema Formation after Cryogenic Brain Injury in Rats

• Baik SW
• Chung KS
• Kim IS
• Kim HK
• Shin SW
• Kim KW

• KMID: 2091503
• DOI: http://doi.org/10.4097/kjae.2000.38.1.152

Abstract

BACKGROUND: After experimental cryogenic cerebral injury, severe focal brain contusion develops due to blood-brain barrier breakdown and vasogenic cerebral edema formation. This study has been conducted to find out the effects of hypertonic saline against cryogenic brain edema in rats.
METHODS
Thirty rats of either sex weighing 250 to 300 g underwent a 60 seconds of cryogenic brain injury. All rats were randomly divided into one of three groups; control group (n = 10), 7.5% saline group (n = 10), and 10% mannitol group (n = 10). The water contents were measured 60 minutes after cryogenic injury by using the dry-weight method.
RESULTS
The water contents in the 7.5% saline and 10% mannitol groups were significantly decreased compared with the control group. The levels of edema in the 7.5% saline and 10% mannitol groups were also significantly decreased compared with the control group. Although it appeared as if that 10% mannitol might decrease edema formation more than 7.5% saline, there were no statistical differences between the 7.5% saline and 10% mannitol groups.
CONCLUSIONS
Hypertonic saline (7.5%) may be as effective agent to reduce edema formation after brain trauma to the same degree as mannitol.

Keyword

Animal: rats; Brain: cryogenic; edema; injury; Fluids: hypertonic saline; mannitol

MeSH Terms

Animals
Blood-Brain Barrier
Brain Edema
Brain Injuries*
Brain*
Edema*
Mannitol*
Rats*
Mannitol