Korean J Cytopathol.  1995 Jun;6(1):1-9.

p 53 Protein Expression in Imprint Cytology of Breast Carcinoma

• Kim DS
• Lee EH
• Kim KK
• Kim MJ
• Lee SJ

Abstract

This study was carried out to determine the usefulness of imprint cytology for detecting p 53 protein in breast carcinoma. NCL-DO7(Novocastra, U.K.) was used to detect p53 protein immunocytochemically. A total of 33 cases was studied. Immunostaining of imprint cytology with NCL-DO7 was positive in 64%(21\33) and showed relatively high coincident rate (80 %) with immunostaining of formalin-fixed, paraffin - embedded specimen. p 53 protein was related to negative estrogen receptor status, but not to the nuclear grade, lymph node metastasis, or tumor size. The fact that p53 protein expression was not related to nuclear grade might be due to predominance of nuclear grade 3. It was easier to determine the nuclear grade is one of the most important prognostic factors, in imprint cytology than in tissue specimen. p53 protein tended to be stained more strongly in imprint cytology than in tissue.

Keyword

Breast carcinoma; Imprint cytology; p53 protein; Immunocytochemistry

MeSH Terms

Breast Neoplasms*
Breast*
Estrogens
Immunohistochemistry
Lymph Nodes
Neoplasm Metastasis
Paraffin
Estrogens
Paraffin