Study on the Immunogistochemical Expression of Placental From Glutathione S-transferase, P53 and CEA in Colorectal Adenomas and Adenocarcinomad - Tumor Marker Study in Colon Cancer Tissues by Immunohistochemical Methods -

Hong, Sook Hee; Choi, Seok Reyol; Kim, Du Hyeong; Han, Sang Young; Roh, Myung Hwan; Huh, Gi Yeong; Shin, Woo Won; Kim, Jong Seong

Abstract

BACKGROUND/AIMS: This study is aimed to observe the expression of GST-z in colorectal adenomas and adenocarcinomas according to the risk and prognostic factors, and investigate the role during carcinogenesis and the possibility of clinical application as a tumor marker of colorectal neoplasm, in cornparision with the expression of p53 protein and CEA.
METHODS
Immuno- histochemical stain was performed in 15 cases of normal colon, 31 adenomas and 63 adenocarci- nomas.
RESULTS
The incidence of GST- expression was higher in adenocarcinoma (95.2%) than normal colon(40%), adenoma(87.1%) and adjacent norrnal mucosa to adenocarcinoma(63.5%) and the degree of the expression was also significantly different among them. In adenomas, the higher the degree of dysplasia, the higher the expression of GST-z. In adenocarcinomas, the expression was significantly decreased in relation with progression to lower histologic grade. The incidence of p53 expression was higher in adenocarcinomas(54.0%) than normal colon(0%), adenoma(25.8%) and adjacent normal mucosa to adenocarcinoma(0%) and the degree of the expression was also significantly different among them. In adenomas, the higher the degree of dysplasia, the higher the expression of p53. In adenocarcinoma, there was no sifnificant difference in relation with the histologic grade and stage. The incidence of CEA expression was higher in adenocarcinoma (96.8%) than normal colon(33.3%), adenoma(64.5%) and adjacent normal mucosa to adenocarci- norna(95.2%) and the degree of the expression was also significantly different among them. In adenomas, there was no significant difference in relation with size, amount of villous component and degree of dysplasia. In adenocarcinomas, the expression was increased in relation with progression to higher stage. The incidence of intense co-expression of GST-z and p53 in adenocarcinoma showed a tendency to be increasing in relation with progression to higher stage. But there was no statistically significant.
CONCLUSIONS
It has been found from the above results that the expression of GST-z is progressively increased during colorectal carcinogenesiser.