Korean J Hematol.
1999 May;34(2):235-240.
Standardization of Cord Blood Separation Method for Establishment of Korean Cord Blood Bank
- Affiliations
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- 1Department of Pediatrics, College of Medicine, Dong-A University, Pusan, Korea.
- 2Department of Pediatrics, College of Medicine, Kye-Myoung University, Korea.
- 3Department of Pediatrics, College of Medicine, Kyung-Pook National University, Taegu, Korea.
- 4Department of Pediatrics, College of Medicine, Chon-Nam National University, Korea.
- 5Department of Pediatrics, College of Medicine, Kwangju, Choong-Nam National University, Korea.
- 6Department of Pediatrics, College of Medicine, Taejon, Catholic University, Korea.
- 7Department of Pediatrics, College of Medicine, Sung-Kyun-Kwan University, Korea.
- 8Department of Pediatrics, College of Medicine, Seoul National University, Korea.
- 9Department of Pediatrics, College of Medicine, Kyung-Hee University, Seoul, Korea.
Abstract
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BACKGROUND: To standardize the separation method of cord blood and establish the Korean Cord Blood Bank, we analysed the data regarding cord blood separation from 8 different hospitals which involved in cord blood banking project.
METHODS
We analysed 306 samples which collected with heparinized syringes and separated within 24 hours by Ficoll-Hypaque density gradient separation method and RBC depletion method with 3% gelatin. We performed the mononuclear cell (MNC) count, viability, CD34+ cell count and CFU-GM count following separation of cord blood. We assessed the yield of hematopoietic stem cells by different separation methods in 9 hospitals and the variability among hospitals which performed cord blood separation by same method.
RESULTS
1) In 204 samples of cord blood separated with Ficoll-Hypaque, there were significant differences in MNC count, viability, CD34+ cell count and CFU-GM count among all engaged hospitals, but not in each individual hospital. 2) In 102 samples of cord blood separated with 3% gelatin, there were significant differences in viability, CFU-GM count and CD34+ cell count among all engaged hospitals, but not in MNC count. 3) The yield of MNCs, CD34+ cells and CFU-GM was higher in the cord blood separated with 3% gelatin than Ficoll-Hypaque.
CONCLUSION
We suggest that RBC depletion method with 3% gelatin would be better than Ficoll-Hypaque method in order to increase the yield of hematopoietic stem cells and standardize the method of cord blood separation.
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