Korean J Obstet Gynecol.  2007 Jun;50(6):901-911.

The relationship between osteoprotegerin (OPG)-receptor activator of NF-kB ligand (RANKL) gene polymorphisms and change in bone mineral density after hormone therapy in postmenopausal Korean women

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea. kimjg@plaza.snu.ac.kr

Abstract


OBJECTIVE
To investigate the relationship of polymorphisms in osteoprotegerin (OPG)-receptor activator of NF-kB ligand (RANKL) gene to changes in circulating OPG and soluble RANKL (sRANKL) levels and in bone mineral density (BMD) after hormone therapy (HT) in postmenopausal Korean women.
METHODS
The OPG T245G, and G1181C polymorphisms and RANKL rs2277438 A/G polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphsim (PCR-RFLP) or direct DNA sequencing in 236 postmenopausal Korean women receiving sequential HT for 1 year. Serum OPG, sRANKL, bone alkaline phosphatase, CrossLaps (CTX), osteocalcin, clacitonin, parathyroid hormone, calicum, and phophorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay and atomic absorptiometry respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry.
RESULTS
The annual percent changes of BMD were not associated with single or combined genotypes of OPG and RANKL gene polymorphisms, and the distributions of these genotypes were not different between HT-responders and HT-nonresponders (women who lose more than 3% of bone mass per year). After HT of 6 months, change in serum sRANKL levels was significantly higher in GG genotype than in other genotype of RANKL gene polymorphism. No differences in the 6 month changes of other bone turnover markers including circulating OPG levels after HT were noted across single OPG genotype and combined genotypes of OPG and RANKL polymorphisms.
CONCLUSIONS
The OPG T245G, and G1181C polymorphisms, and RANKL polymorphism did not associate with change in BMD after HT in postmenopausal Korean women, and RANKL polymorphism affects change in circulating sRANKL levels after HT.

Keyword

OPG; RANKL; Gene polymorphism; BMD; HT

MeSH Terms

Absorptiometry, Photon
Alkaline Phosphatase
Bone Density*
Enzyme-Linked Immunosorbent Assay
Female
Femur
Genotype
Humans
Immunoassay
NF-kappa B*
Osteocalcin
Osteoprotegerin*
Parathyroid Hormone
Sequence Analysis, DNA
Spine
Alkaline Phosphatase
NF-kappa B
Osteocalcin
Osteoprotegerin
Parathyroid Hormone
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