Korean J Obstet Gynecol.  1997 Jul;40(7):1348-1353.

The Effect of Free Radical Scavenger, Superoxide Dismutase ( SOD ) in the Early Embryo Culture of Mice

• Kim MR
• Kwon HC
• Ryu HS
• Hwang KJ
• Park JY
• Hong SJ
• Lee YD
• Gye MC
• Choi KW
• Oh KS

Abstract

It is well known that developmental delay or arrest occurs before implantation in mamals, which have undergone in vitro culture. Recently, these phenomena are being attributed to oxygen free radicals, and successful cell culture are being obtained by lowering the oxygen environment of in vitro culture. This is due to the fact that the oxygen concentration in the fallopian tube is around 5%, which is lower than the room air 20% concentraition for in vitro culture. Superoxide dismutase(SOD), which are free radical scavenger, were added to early embryo mice culture and compared the free radical level at each stage of development with that of culture in which free radical scavenging agent was not added. The highest free radical level in control group observed in the late 2-cell stage and was markedly decreased if SOD added to culture media. Blastulation rate, hatching rate, and blastomere count as the parameters of embryo development was significantly increased in the experimental group. This study suggests the possibility of improvement in in-vitro embryo culture with media including free radical scavengers.

Keyword

Oxygen free radicals; Superoxide dismutase ( SOD ); Free radical scavenger

MeSH Terms

Animals
Blastomeres
Cell Culture Techniques
Culture Media
Embryonic Development
Embryonic Structures*
Fallopian Tubes
Female
Free Radical Scavengers
Free Radicals
Mice*
Oxygen
Pregnancy
Superoxide Dismutase*
Superoxides*
Culture Media
Free Radical Scavengers
Free Radicals
Oxygen
Superoxide Dismutase
Superoxides