Korean J Physiol Pharmacol.
1999 Dec;3(6):571-578.
Effects of protein kinase C modulation on hepatic hemodynamics and glucoregulation
- Affiliations
-
- 1Department of Physiology, Yonsei University, Wonju College of Medicine, Wonju, Korea.
- 2Department of Institute of Medical Engineering, Yonsei University, Wonju College of Medicine, Wonju 220-701, Korea.
- 3Department of Physiology, Stritch School of Medicine, Loyola university of Chicago 2160 South First Avenue Maywood, IL 60153, USA.
Abstract
- This study evaluated the effects of PKC activation using phorbol 12-myristate 13-acetate (PMA) and PKC inhibition using the isoquinoline sulfomide derivative H-7 on hemodynamics and glucoregulation in the isolated perfused rat liver. Livers were isolated from fed male Holtzman rats and perfused with Krebs Ringer bicarbonate solution under a constant flow of 50 ml/min at 35degreeC. Portal vein pressure, glucose and lactate concentrations in the medium and oxygen consumption rates were continuously monitored by a Grass polygraph, YSI glucose and lactate monitors, and a YSI oxygen monitor, respectively. PMA at concentration of 2 to 200 nM increased the portal vein pressure, glucose and lactate production, but decreased oxygen consumption rate in a dose-dependent fashion. H-7 (200 micrometer) attenuated PMA (50 nM)-induced vasoconstriction (15.1+/-1.36 vs 10.56+/-1.17 mmHg), glucose production rate (91.3+/-6.15 vs 71.8+/-2.50 micromoles/g/hr), lactate production rate (72.4+/-6.82 vs 53.6+/-4.82 micromoles/g/hr) and oxygen consumption rate (33.7+/-1.41 vs 27.9+/-1.75 microliter/g/min). The effects of PMA were blocked either by addition of verapamil (9 micrometer) or perfusion with Ca2+-free KRB. These results suggest that the hemodynamic and glucoregulatory changes in the perfused rat liver are mediated by protein kinase C activation and require Ca2+ influx from the extracellular fluid.
Keyword
MeSH Terms
-
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Animals
Extracellular Fluid
Glucose
Hemodynamics*
Hepatocytes
Humans
Lactic Acid
Liver
Male
Oxygen
Oxygen Consumption
Perfusion
Poaceae
Portal Vein
Protein Kinase C*
Protein Kinases*
Rats
Rats, Sprague-Dawley
Vasoconstriction
Verapamil
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Glucose
Lactic Acid
Oxygen
Protein Kinase C
Protein Kinases
Verapamil
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Regulation of Protein Kinase in KCl-induced Contraction of Cat Gastric Smooth Muscle
- Role of Protein Kinase in Spontaneous Phasic Contraction of Cat Gastric Smooth Muscle
- Role of Protein Kinase C in Normal and Preconditioned Myocardium
- Effects of psychotropic drugs on phospholipase C and protein kinase C in vitro
- The Effects of Chronic Carbamazepine Administration on Protein Kinase A and Protein Kinase C Activities in Rat Brain
