Yonsei Med J.  2014 May;55(3):576-583. 10.3349/ymj.2014.55.3.576.

Expression of Glycolysis-Related Proteins in Solid Papillary Carcinoma of the Breast According to Basement Membrane Status

• Kwon JE
• Jung WH
• Koo JS

• KMID: 2068665
• DOI: http://doi.org/10.3349/ymj.2014.55.3.576

Abstract

PURPOSE
The aim of this study was to investigate the differences of expression in glycolysis-related proteins such as Glut-1, carbonic anhydrase (CA) IX, and monocarboxylate transporter (MCT) 4 according to the myoepithelial cell (MEC) and basement membrane (BM) status in solid papillary carcinoma (SPC) of the breast.
MATERIALS AND METHODS
Immunohistochemical evaluation of Glut-1, CAIX, and MCT4, as well as p63 and type IV collagen, were performed on 23 SPC cases.
RESULTS
Six and nine cases of SPC showed the presence and absence of myoepithelial cells, respectively, and eight cases belonged to the borderline status (p63-positive MEC on some areas of the outer tumor surface but not in others). BM was partially or completely absent in 14 cases and present in nine cases. SPC lacking BM more frequently showed high expression of CAIX than SPC with BM (p=0.037).
CONCLUSION
In SPC of the breast, a strong expression of CAIX seems to be associated with an increasing degree of loss of BM, which can be interpreted as BM degradation due to the induction of extracellular acidity with increasing expression of CAIX.

Keyword

Basement membrane; breast; metabolism; myoepithelial cell; solid papillary carcinoma

MeSH Terms

Adult
Aged
Basement Membrane/*metabolism
Breast Neoplasms/*metabolism
Carcinoma, Papillary/*metabolism
Excitatory Amino Acid Transporter 2/metabolism
Female
Glycolysis
Humans
Immunohistochemistry
Middle Aged
Monocarboxylic Acid Transporters/metabolism
Muscle Proteins/metabolism
Tumor Markers, Biological/*metabolism
Excitatory Amino Acid Transporter 2
Monocarboxylic Acid Transporters
Muscle Proteins
Tumor Markers, Biological

Figure

• 

  Fig. 1 Expression of glycolysis-related proteins according to myoepithelial cell and basement membrane status. In situ SPC shows p63-positive myoepithelial cells in the outer surface of the tumor nodule (arrow) and a basement membrane that is positive for type IV collagen (arrow). Indefinite SPC shows p63 negativity on one side and p63-positive myoepithelial cells at the outer surface of the other side of the tumor nodule and is negative for type IV collagen. In invasive SPC, both p63 and type IV are negative at the outer surface of the tumor nodule. SPC which is negative for type IV collagen shows a high level of CAIX expression, and invasive carcinoma shows high level of MCT4 expression in the stroma (S) as well as tumor cells (T). SPC, solid papillary carcinoma; MCT, monocarboxylate transporter; CAIX, carbonic anhydrase IX.

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