Korean J Urol.  2005 Apr;46(4):327-335.

Analysis of Matrix Metalloproteinase Expression in the Recurrence of Superficial Low Grade Urothelial Bladder Cancer

• Choi YD
• Ahn HS
• Cho NH
• Lee MK
• Kang SH
• Yang WJ

Abstract

PURPOSE: Superficial bladder transitional cell carcinomas (TCCa) have high recurrence rates following endoscopic resection. Most of the parameters used for the prediction of a recurrence are associated with high grade TCCa. However, there are few valid parameters for prediction of the recurrence of low grade TCCa. We aimed to elucidate the prime factor of a recurrence of superficial low grade bladder TCCa.
MATERIALS AND METHODS
Thirty cases of superficial low grade bladder TCCa were analyzed, 15 of which were recurred cases. Fresh frozen tumor and normal tissues were used for the analysis. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots analyses were subsequently performed to evaluate the validity of the molecules identified by a 0.12K cDNA array, including matrix metalloproteinase (MMP), oncogenes and cell-cycle-related genes.
RESULTS
On cDNA microarray analysis, MMP-1, 2, 9, 12 and 15, and transforming growth factor beta1 (TGFbeta1), vascular endothelial growth factor (VEGF) and fos were found to be significantly upregulated in the recurred cases. From the real-time RT-PCR for the validation of those molecules, MMP-1 and 12, and TGFbeta1, VEGF and fos were significantly overexpressed in the recurred cases. Western blot showed significant overexpression of MMP-1 and 12, and TGFbeta1, VEGF and fos in the recurred cases.
CONCLUSIONS
The overexpressions of MMP-1 and 12, and TGFbeta1, VEGF and fos may be associated with the recurrence of superficial low grade bladder TCCa.

Keyword

Bladder tumor; Superficial; Recurrence; RT-PCR; Matrix metalloproteinases

MeSH Terms

Blotting, Western
Carcinoma, Transitional Cell
Matrix Metalloproteinases
Oligonucleotide Array Sequence Analysis
Oncogenes
Recurrence*
Reverse Transcriptase Polymerase Chain Reaction
Transforming Growth Factor beta1
Urinary Bladder Neoplasms*
Urinary Bladder*
Vascular Endothelial Growth Factor A
Matrix Metalloproteinases
Transforming Growth Factor beta1
Vascular Endothelial Growth Factor A