Obstet Gynecol Sci.  2015 Sep;58(5):333-339. 10.5468/ogs.2015.58.5.333.

Placental expression of D6 decoy receptor in preeclampsia

• Cho GJ
• Lee ES
• Jin HM
• Lee JH
• Kim YS
• Oh MJ
• Seol HJ
• Hong SC
• Kim HJ

• KMID: 2058455
• DOI: http://doi.org/10.5468/ogs.2015.58.5.333

Abstract

OBJECTIVE
The purpose of this study was to investigate the expression of the D6 decoy receptor that can bind chemokines and target them for degradation, resulting in inhibition of inflammation in placentas from preeclamptic and normal pregnancies.
METHODS
The current study was carried out in 35 pregnant women (23 patients with preeclampsia and 12 healthy, normotensive pregnant women) during the third trimester of pregnancy. The expressions of D6 decoy receptor in the placenta were determined with real time reverse transcriptase polymerase chain reaction and western blotting.
RESULTS
The mRNA and protein of D6 decoy receptor were detected in all of placentas from preeclamptic and normal pregnancies. Placental D6 decoy receptor mRNA expression was significantly lower in patients with preeclampsia than in patients with normal pregnancies. Western blot analyses revealed decreased protein expression in cases of preeclampsia.
CONCLUSION
The expression of the D6 decoy receptor in preeclamptic placentas was significantly lower than in normal placentas. Further studies are needed to clarify the underlying mechanisms that link decreased expression of placental D6 decoy receptor and preeclampsia.

Keyword

D6 decoy receptor; Placenta; Preeclampsia

MeSH Terms

Blotting, Western
Chemokines
Female
Humans
Inflammation
Placenta
Pre-Eclampsia*
Pregnancy
Pregnancy Trimester, Third
Pregnant Women
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Chemokines
RNA, Messenger

Figure

• 

  Fig. 1 Placental expression of D6 decoy receptor (white arrows) by immunofluorescence in normal (A) and preeclamptic (B) placenta (×200).

• 

  Fig. 2 mRNA expression of the placental D6 decoy receptor in preeclamptic and normal placentas. Expression was normalized using the GAPDH housekeeping gene product as an endogenous reference. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.

• 

  Fig. 3 Western blot analysis for protein expression of placental D6 decoy receptor from preeclamptic and normal placentas. (A) Results from a representative experiment. Bands of almost identical intensity for GAPDH are also shown for each experiment. (B) Mean±standard error of the mean of densitometric numbers was calculated by densitometry. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.

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