Biomol Ther.  2013 Sep;21(5):391-397.

Antinociceptive, Immunomodulatory and Antipyretic Activity of Nymphayol Isolated from Nymphaea stellata (Willd.) Flowers

• Pandurangan SB
• Paul AS
• Savarimuthu I
• Ali AA

Abstract

In the present study, we aimed to analyze the antinociceptive, immunomodulatory and antipyretic activities of nymphayol were investigated in wistar rats and mice. Antinociceptive effect was evaluated by acetic acid induced writhing, formalin induced paw licking and hot-plate tests. Immunomodulatory activity was assessed by neutrophil adhesion test, humoral response to sheep red blood cells, delayed-type hypersensitivity, phagocytic activity and cyclophosphamide induced myelosuppression. Antipyretic activity was evaluated by yeast induced hyperthermia in rats. Nymphayol produced significant (p<0.05) antinociceptive activity in acetic acid induced writhing response and late phase of the formalin induced paw licking response. Pre-treatment with nymphayol (50 mg/kg, oral) evoked a significant increase in neutrophil adhesion to nylon fibres. The augmentation of humoral immune response to sheep red blood cells by nymphayol (50 mg/kg) was evidenced by increase in antibody titres in rats. Oral administration of nymphayol (50 mg/kg) to rats potentiated the delayed-type hypersensitivity reaction induced by sheep red blood cells. Treatment with nymphayol showed a significant (p<0.05) reduction in pyrexia in rats. The results suggest that nymphayol possesses potent anti-nociceptive, immunomodulatory and antipyretic activities.

Keyword

Antinociceptive; Immunomodulatory; Antipyretic; Nymphaea stellata; Nymphayol; Writhing response

MeSH Terms

Acetic Acid
Administration, Oral
Animals
Cyclophosphamide
Erythrocytes
Fever
Flowers*
Formaldehyde
Hypersensitivity
Hyperthermia, Induced
Immunity, Humoral
Mice
Neutrophils
Nylons
Nymphaea*
Rats
Rats, Wistar
Sheep
Yeasts
Acetic Acid
Cyclophosphamide
Formaldehyde
Nylons