Ann Lab Med.  2015 Jul;35(4):454-457. 10.3343/alm.2015.35.4.454.

Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests

• Heraud S
• Freydiere
• Doleans-Jordheim A
• Bes M
• Tristan A
• Vandenesch F
• Laurent F
• Dauwalder

• KMID: 2045870
• DOI: http://doi.org/10.3343/alm.2015.35.4.454

Abstract

Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.

Keyword

Staphylococcus aureus; Methicillin resistance; Blood culture; Accuracy; Immunochromatographic test

MeSH Terms

Bacteremia
Diagnosis
Gram-Positive Cocci
Methicillin Resistance
Methicillin*
Methicillin-Resistant Staphylococcus aureus
Mortality
Staphylococcus aureus*
Methicillin

Figure

• 

  Fig. 1 Example of false positive Binax Now Staphylococcus aureus test with charcoal particles in Bact/ALERT bottles.

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