J Korean Assoc Maxillofac Plast Reconstr Surg.  2005 May;27(3):205-217.

The expression of TGF-beta1, IGF-I, bFGF in distraction osteogenesis according to different distraction rates in rabbit's mandible

Affiliations
  • 1Department of Dentistry, St. Vincent's Hospital, The Catholic University of Korea, 93 Ji-Dong, Paldal-Ku, Suwon, Korea. omsjyj@kornet.net

Abstract

Distraction osteogenesis is a technique of lengthening bone including soft tissue by gradual separation of surgically divided bone surfaces. Although the biomechanical, histological, and ultrastructural changes associated with distraction osteogenesis have been widely described, the molecular mechanisms governing the formation of new bone in distracted bone segments remain largely unclear. However, such information has significant clinical implications because it may enable targeted therapeutic manipulations designed to The purpose of this study was to evaluate the expression of TGF-beta1, IGF-I and bFGF in distraction When twenty-four adult rabbits underwent open osteotomy between the premolar and mental foramen, an external bilateral distraction device was applied. Latency was allowed for five days before distraction. Three different distraction rates were 0.7 mm/day (A, n=8), 1.4 mm/day (B, n=8) and 2.4 mm/day (C, n=8). The distraction device was activated with the same distraction rhythms of twice a day until 4.9 mm (A and B group) and 8.4 mm (C group) length gains was achieved. The animals were sacrificed at postoperative 3, 7, 14 and 28 days. The bony specimens were stained with HandE for histologic examination, and RT-PCR analysis was done for the identification of the expression of TGF-beta1, IGF-I and bFGF. The 0.7 mm/day and 1.4 mm/day distraction rate groups were shown to improve regenerative bone formation on radiographic and histologic examination. Also, TGF-beta1, IGF-I and bFGF expression increased in the 0.7 mm/day and 1.4 mm/day distraction rate groups. But the 2.4 mm/day distraction rate group specimen was different with adjacent normal bone and hardly expressed of growth factors. These findings suggest that improved new bone formation in the 0.7 mm/day and 1.4 mm/day distraction rates is associated with enhanced expression of TGF-beta1, IGF-I and bFGF by mechanical tension stress. Additionally, the 0.7 mm/day and 1.4 mm/day distraction rate groups were significantly different from the According to the above results, it seems possible to apply a distraction rate of up to 1.4 mm/day a day in rabbit's mandible. And further studies are needed to evaluate growth factors of TGF-beta1 and IGF-I, which are excellent in expression.

Keyword

Distraction osteogenesis; Distraction rate; Growth factors; Gene expression

MeSH Terms

Adult
Animals
Bicuspid
Gene Expression
Hand
Humans
Insulin-Like Growth Factor I*
Intercellular Signaling Peptides and Proteins
Mandible*
Osteogenesis
Osteogenesis, Distraction*
Osteotomy
Rabbits
Transforming Growth Factor beta1*
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Transforming Growth Factor beta1
Full Text Links
  • JKAMPRS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error