Korean J Blood Transfus.  2003 Dec;14(2):153-159.

Evaluation of New Third Generation LG Anti-HIV 1/2 Plus ELISA Kit

• Yeom JS
• Ryu SH
• Kang HJ
• Park JW

Abstract

BACKGROUND: In several studies, failure of some of the previously developed anti-HIV-1/HIV-2 ELISA tests to detect HIV-1 group O infected blood had been reported. All of the anti-HIV-1/HIV-2 ELISA tests made by Korean manufactures have limitation in detecting HIV-1 group O infected blood. Newly developed LG Anti-HIV 1/2 Plus is the first Korean made product to overcome this limitation.
METHODS
Sensitivity and specificity of LG Anti-HIV 1/2 Plus (LG Life Science, Seoul, Korea) and Enzygnost Anti-HIV 1/2 Plus (Dade Behring, Marburg, Germany) were evaluated using 82 Anti-HIV 1 positive samples, 2 Anti-HIV 1 group O positive samples, 25 Anti-HIV 2 positive samples and commercial BBI (Boston Biomedica, Inc., MA, USA)) Anti-HIV panels and 382 Anti HIV negative samples (300 healthy donors, 13 alcoholics sera, 20 end-stage renal disease sera, 20 HBsAg positive sera, 22 anti-HBs antibody positive sera, 7 anti-HCV positive sera) respectively. Seroconversion window was evaluated using 2 BBI(R) seroconversion panel. Intrapersonal and interpersonal reproducibility of the test were evaluated also.
RESULTS
Sensitivity and specificity of both tests were 100%. Analysis of window period using commercial seroconversion panel showed no difference between both tests.
CONCLUSION
LG Anti-HIV 1/2 Plus LISA showed excellent sensitivity and specificity in tested samples including HIV-1 group O infected sera. It can be concluded that LG Anti-HIV 1/2 Plus ELISA is capable of detecting most of the HIV-1, HIV-2 type and it could be used in screening donated blood.

Keyword

Anti-HIV; Enzyme-linked immunosorbent assay; Sensitivity and specificity

MeSH Terms

Alcoholics
Biological Science Disciplines
Enzyme-Linked Immunosorbent Assay*
Hepatitis B Surface Antigens
HIV
HIV-1
HIV-2
Humans
Kidney Failure, Chronic
Mass Screening
Sensitivity and Specificity
Seoul
Tissue Donors
Hepatitis B Surface Antigens