Yeungnam Univ J Med.  1998 Jun;15(1):143-150. 10.12701/yujm.1998.15.1.143.

Comparison of Three Third-generation Anti-HCV Enzyme Immunoassay Tests

• Cho HS
• Moon JY
• Lee CH
• Kim KD

• KMID: 2154419
• DOI: http://doi.org/10.12701/yujm.1998.15.1.143

Abstract

The aim of this study was to evaluate domestic enzyme immunoassay(EIA) kit ?LG HCD 3.0?(LG) for the detection of antibody to hepatitis C virus(anti-HCV) in comparision with Axsym HCV version 3.0(Axsym), Cobas Core anti-HCV EIA(Cobas). Cobas kit shows better clear distinction between positive and negative by signal/cutoff ratio(S/C), but it also reveal relatively high false positive rate. The concordance rate of test results between LG and Axsym was 96.2%, between LG and Cobas was 95.5%, and total agreement between three EIA kit was 93.9%. LG were relative poor distinction between positive and negative results, but it could be applied clinically as a screening tool for hepatitis C in general population. The S/C of one false negative result by LG was 0.91, and false positive were less than 4.0, therefore we concluded it is necessary to confirm by immunoblotting assay when S/C were between 0.8 and 4.0.

Keyword

Hepatitis C virus; Anti-HCV; Third-generation Enzyme Immunoassay

MeSH Terms

Hepacivirus
Hepatitis C
Immunoblotting
Immunoenzyme Techniques*
Mass Screening