Korean J Gastroenterol.  2002 Apr;39(4):260-268.

The Promising Effect of Egg Yolk Antibody (Immunoglobulin Yolk) on the Treatment of Helicobacter pylori-associated Gastric Diseases

• Roe IH
• Nam SW
• Yang MR
• Myung NH
• Kim JT
• Shin JH

Abstract

BACKGROUND/AIMS: The importance of eggs as a source of specific antibodies (IgY) is well recognized. We investigated the efficacy of specific anti-Helicobacter pylori (H. pylori) IgY antibody against H. pylori infection.
METHODS
Specific anti-H. pylori IgY was obtained from the hens which were immunized with whole cell lysate (200ng/mL) of H. pylori. Specific IgY was recognized by SDS-PAGE and the activity was determined by ELISA. After administration of specific IgY, the growth changes of H. pylori, the response of H. pylori-infected KATO III and AGS cell, and in vivo effect of IgY on H. pylori-infected gerbil model were determined.
RESULTS
Specific IgY inhibited the growth of H. pylori by 90% within 6 hours in contrast to no inhibitory effect on the growth of E. coli or L. salivarius. After treating with 10 mg/mL of specific IgY, H. pylori-infected (10(7) CFU/mL) KATO III and AGS cell did not proliferate. Bacterial adhesion was definitely decreased in H. pylori-infected AGS cell treated with 10 mg/mL of IgY. Oral administration of 10 mg/mL of IgY daily for 30 days remarkably inhibited gastric inflammation in H. pylori-infected gerbil, whereas 1 mg/mL of IgY did not reduce gastric inflammation.
CONCLUSIONS
Specific anti-H. pylori IgY might provide promising effects on the H. pylori-associated gastric diseases.

Keyword

Specific anti-H. pylori IgY; H. pylori-associated gastric diseases

MeSH Terms

Administration, Oral
Antibodies
Bacterial Adhesion
Egg Yolk*
Eggs
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Gerbillinae
Helicobacter*
Inflammation
Ovum*
Stomach Diseases*
Antibodies