Korean J Obstet Gynecol.  1998 Nov;41(11):2821-2826.

Increased Expression of Prostaglandin H synthase by Aspirin in Cultured Cells from Amnionic Cell Line WISH Cells

• Cha MS
• Kwak JY

Abstract

Prostaglandins which are produced from amnionic cells are known to play a major role in uterine contraction and cervical dilatation in human. Recently it is reported that aspirin increases the expression of PGHS-1 and PGHS-2 in trophoblast cells from placenta. We examined here the changes of immunoreactive prostaglandin H synthase (PGHS) level by aspirin in cultured cells from amniotic fluid and human amnionic WISH cells. PMA (10-7 M), an activator of protein kinase C increased the induction of PGHS-2 in both cells with or without fetal calf serum. PGHS-2 protein was also increased significantly by 10-4 M aspirin at 6 hours in both cells in the presence of serum but it was not increased in the absence of serum. The expression of PGHS-1 protein was enhanced by asprin but not by PMA in the absence of serum. Other anti-inflammatory drugs such as acetaminophen, indomethacin, dexame- thasone, and mefenamic acid increased the PGHS-2 protein level in WISH cells. PMA-induced PGHS-2 expression in WISH cells was not decreased by aspirin, on the contrary, the level was increased additively. Our results show that the increased expression of PGHS in amnion cells or other amniotic fluid cells by aspirin and other several anti-inflammatory drugs is through an unidentified effect rather than feedback effect by depletion of prostaglandin.

Keyword

Prostaglandin H synthase (PGHS); Amniotic cell; WISH cell; Aspirin

MeSH Terms

Acetaminophen
Amnion*
Amniotic Fluid
Aspirin*
Cell Line*
Cells, Cultured*
Cyclooxygenase 2
Female
Humans
Indomethacin
Labor Stage, First
Mefenamic Acid
Placenta
Pregnancy
Prostaglandin-Endoperoxide Synthases*
Prostaglandins
Protein Kinase C
Trophoblasts
Uterine Contraction
Acetaminophen
Aspirin
Cyclooxygenase 2
Indomethacin
Mefenamic Acid
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Protein Kinase C