J Korean Ophthalmol Soc.  2003 May;44(5):1180-1187.

The Effect of Vascular Endothelial Growth Factor on Vascularization in Porous Polyethylene Orbital Implant (Medpor(R))

• Choi YS
• Yim HB
• Choi WC

Abstract

PURPOSE
We investigated the effect of VEGF usage and the method of surgery on the vascularization rate of the porous orbital implant (Medpor(R)). METHODS: Thirty six Newzealand white rabbits were randomized into 2 groups according to the method of surgery (evisceration and intrascleral implantation and evisceration and retroscleral implantation). Each group was subdivided into two groups, a group treated with VEGF, and the other without VEGF treatment. The degree of vascularization was observed in the four groups at 1, 2, 4 weeks by using cryofilm transfer kit (Finetec, Tokyo, Japan). The implant was sliced at the equator with cryomicrotome and stained with Hematoxylin and Eosin, Masson's trichrome and reticulin. The sample was observed to assay the degree of fibrovascular ingrowth with light microscope. RESULTS: The group in which VEGF was used and the implant was inserted retrosclerally (n=9) showed significantly higher vascularization rate than the other three groups (P<0.05). Vascularization rate of the implant was significantly higher in the group in which VEGF was used (n=18). The rate was 83.6% compared to 64.2% in the control group (P<0.05). Different methods of surgery also showed significant difference. The rate of vascularization was 64.8% in the intrascleral inserted group (n=18) compared to 82.9% in the retroscleral inserted group (P<0.05). However, there was no difference in the vasculariztion rate at different time of observation (p>0.05). CONCLUSIONS: After using VEGF or inserting the implant retrosclerally, the vascularization of the implant was significantly incresed.

Keyword

Medpor(R); VEGF (vascular endothelial growth factor); Vascularization rate

MeSH Terms

Eosine Yellowish-(YS)
Hematoxylin
Orbit*
Orbital Implants*
Polyethylene*
Rabbits
Reticulin
Vascular Endothelial Growth Factor A*
Eosine Yellowish-(YS)
Hematoxylin
Polyethylene
Reticulin
Vascular Endothelial Growth Factor A