Exp Neurobiol.  2014 Mar;23(1):86-92. 10.5607/en.2014.23.1.86.

Melittin Ameliorates the Inflammation of Organs in an Amyotrophic Lateral Sclerosis Animal Model

• Lee SH
• Choi SM
• Yang EJ

• KMID: 2009028
• DOI: http://doi.org/10.5607/en.2014.23.1.86

Abstract

Amyotrophic lateral sclerosis (ALS) is a devastating progressive neurodegenerative disorder characterized by a selective loss of motor neurons in the spinal cord, brainstem, and motor cortex, leading to weakness of the limb and bulbar muscles. Although the immediate cause of death in ALS is the destruction of motor neurons, ALS is a multi-organ disease that also affects the lungs, spleen, and liver. Melittin is one of components of bee venom and has anti-neuroinflammatory effects in the spinal cord, as shown in an ALS animal model. To investigate the effects of melittin on inflammation in the lungs and spleen, we used hSOD1(G93A) transgenic mice that are mimic for ALS. Melittin treatment reduced the expression of inflammatory proteins, including Iba-1 and CD14 by 1.9- and 1.3-fold (p<0.05), respectively, in the lungs of symptomatic hSOD1(G93A) transgenic mice. In the spleen, the expression of CD14 and COX2 that are related to inflammation were decreased by 1.4 fold (p<0.05) and cell survival proteins such as pERK and Bcl2 were increased by 1.3- and 1.5-fold (p<0.05) in the melittin-treated hSOD1G93A transgenic mice. These findings suggest that melittin could be a candidate to regulate the immune system in organs affected by ALS.

Keyword

Amyotrophic lateral sclerosis (ALS); hSOD1(G93A); Melittin; inflammation

MeSH Terms

Amyotrophic Lateral Sclerosis*
Animals*
Bee Venoms
Brain Stem
Cause of Death
Cell Survival
Extremities
Immune System
Inflammation*
Liver
Lung
Melitten*
Mice
Mice, Transgenic
Models, Animal*
Motor Cortex
Motor Neurons
Muscles
Neurodegenerative Diseases
Spinal Cord
Spleen
Bee Venoms
Melitten