J Korean Soc Microbiol.  2000 Feb;35(1):87-95.

Apoptosis induction and associated factor of Staphylococcus aureus in J774A.1 mouse macrophage cell line

• Kim SH
• Lee CM
• Jeong SJ
• Jeong MH
• Kim JK
• Cha JK
• Lee HS
• Lim YJ
• Lee SH

Abstract

Staphylococcus aureus infections are often life-threatening. Relatively little is known about the host response to these infections, in particular, the implication of apoptosis induced by this microorganism. In this study, we have shown that S. aureus was cytotoxic to J774A.1 cell, a murine macrophage cell line. The cell death mediated by S. aureus occurred through apoptosis, as shown by increase in the proportion of fragmented host cell DNA. Although phagocytosis and NO production had important role in the induction of apoptosis, the contact between bacteria and host cells was not essential for this pathway. A certain bacterial product could also induce typical caspase-dependent apoptosis of J774A.1 cell. It is expected that new interpretation may be possible to host-parasite relationship based on these results.

MeSH Terms

Animals
Apoptosis*
Bacteria
Cell Death
Cell Line*
DNA
Host-Parasite Interactions
Macrophages*
Mice*
Phagocytosis
Staphylococcus aureus*
Staphylococcus*
DNA