J Korean Acad Prosthodont.  2013 Jul;51(3):175-182. 10.4047/jkap.2013.51.3.175.

The effect of alendronates administration duration on the healing of extraction socket in rats: pilot study

• Kim JH
• Hong JH
• Choi HM
• Park YB
• Moon HS

• KMID: 2000166
• DOI: http://doi.org/10.4047/jkap.2013.51.3.175

Abstract

PURPOSE
The purpose of this study was to investigate the effect of different administration duration of alendronate on initial wound healing and new bone formation of extraction socket in rats.
MATERIALS AND METHODS
Fifteen male Sprague-Dawley rats (body weight 130-140 g, 4 weeks old, male) were divided into control group (no alendronate administration) and experimental group (alendronate administration). Experimental group was subdivided into 1 week administrated group, 2 week administrated group, 4 week administrated group and 6 week administrated group according to duration of administration. For the experimental groups, during the designated time period (at the time of extraction, 1 week before extraction, 3 week before extraction and 5 week before extraction) till 1 week after extraction, rats were subcutaneously injected with Alendronate at the dose of 1.0 mg/Kg three times a week. Each specimen from 6 week experimental group and control group were used for microarray analysis, and other specimens were used for histological analysis. The rate of new bone formation within the extraction site and bone loss activity was analyzed using TRAP staining. Statistical analysis was performed using Kruskal Wallis test. (alpha=.05)
RESULTS
After one week from the time of extraction, the rate of new bone formation within extraction site for the control group (16.77% +/- 1.36%) compared to the 4 week experimental group (14.99% +/- 6.26%) was lower. However, no statistically significant difference was found. Increase in the number of inactive lacuna (empty lacuna) and decrease in the number of TRAP positive cell were identified with increased duration of administration. There was no significant difference.
CONCLUSION
The results of this study showed as the duration of Alendronate administration increased the rate of new bone formation decreased with loss of bone activity and reduced number of osteoclast.

Keyword

Alendronate; Bisphosphonate; Extraction socket; bisphosphonate related osteonecrosis of jaw; Wound healing

MeSH Terms

Alendronate
Animals
Humans
Male
Microarray Analysis
Osteoclasts
Osteogenesis
Pilot Projects
Rats
Rats, Sprague-Dawley
Wound Healing
Alendronate

Figure

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  Fig. 1. Extraction socket of the first molar of the rat. A: Right maxillary first molar of the rat, B: Extraction socket C: Extracted maxillary first molar.

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  Fig. 2. A: Interested area of extraction socket (H&E stain ×12.5), B: Border of mesial root (H&E stain ×50) - Image Pro plus program version 4.5 software (Media cybernetics Inc, USA).

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  Fig. 3. The selected area for measurement in the extracted socket of mesial root. A: Extracted socket area of the mesial root of the maxillary first molar, B-D: 3 lateral areas beside the mesial root, E: Septum area. Each blue square (300 ㎛× 300 ㎛).

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  Fig. 4. Extraction socket areas of the each group. Left: H&E, ×12.5, Right: H&E, ×50)

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  Fig. 5. Empty lacunae counting (Left: H&E, ×100): White arrows indicate empty lacunae.

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  Fig. 6. TRAP positive cell counting (Right: TRAP, ×200): White arrows indicate TRAP positive cells.

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