J Korean Acad Periodontol.  2008 Dec;38(4):679-690. 10.5051/jkape.2008.38.4.679.

Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface

• Lee KW
• Kim TI
• Seol YJ
• Lee YM
• Ku Y
• Rhyu IC
• Chung CP
• Han SB

• KMID: 1999600
• DOI: http://doi.org/10.5051/jkape.2008.38.4.679

Abstract

PURPOSE
The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). MATERIAL AND METHODS: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 microgram/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done.
RESULTS
Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days.
CONCLUSION
Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.

Keyword

Enamel matrix derivatives; PDL cell; Tetracycline; EDTA

MeSH Terms

Alkaline Phosphatase
Cell Count
Dental Enamel
Dentin
Edetic Acid
Electronics
Electrons
Humans
Periodontal Ligament
Tetracycline
Tooth
Alkaline Phosphatase
Edetic Acid
Tetracycline