Korean J Vet Res.
2013 Jun;53(2):95-101.
Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection
- Affiliations
-
- 1College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. twhahn@kangwon.ac.kr
- 2College of Pharmacy, Kangwon National University, Chuncheon 200-701, Korea.
- 3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.
Abstract
- The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Protection Against Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Enteritidis Infection in Layer Chickens Conferred by a Live Attenuated Salmonella Typhimurium Strain
- Salmonella Serovars from Foodborne and Waterborne Diseases in Korea, 1998-2007: Total Isolates Decreasing Versus Rare Serovars Emerging
- Prevalence of Salmonella Serovars Isolated from Domestic Residents and Overseas Travelers in Korea, 2004~2005
- A Virulent Salmonella enterica Serovar Enteritidis Phage SE2 with a Strong Bacteriolytic Activity of Planktonic and Biofilmed Cells
- Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis Identified by Multiplex PCR in Korea
