J Korean Acad Conserv Dent.  2009 Sep;34(5):406-414. 10.5395/JKACD.2009.34.5.406.

The effect of concentration and application time of hydrogen peroxide on the microtensile bond strength of resin restorations to the dentin at different depths

• Son JL
• Lee GY
• Kang YM
• Oh YT
• Lee KW
• Kim TG

• KMID: 1986603
• DOI: http://doi.org/10.5395/JKACD.2009.34.5.406

Abstract

The purpose of this study was to examine the effect of hydrogen peroxide at different application time and concentrations on the microtensile bond strength of resin restorations to the deep and the pulp chamber dentin. A conventional endodontic access cavity was prepared in each tooth, and then the teeth were randomly divided into 1 control group and 4 experimental groups as follows: Group 1, non treated; Group 2, with 20% Hydrogen peroxide(H2O2); Group 3, with 10% H2O2; Group 4, with 5% H2O2; Group 5, with 2.5% H2O2; the teeth of all groups except group 1 were treated for 20, 10, and 5min. The treated teeth were filled using a Superbond C&B (Sun medical Co., Shiga, Japan). Thereafter, the specimens were stored in distilled water at 37degrees C for 24-hours and then sectioned into the deep and the chamber dentin. The microtensile bond strength values of each group were analyzed by 3-way ANOVA and Tukey post hoc test(p < 0.05). In this study, the microtensile bond strength of the deep dentin (D1) was significantly greater than that of the pulp chamber dentin (D2) in the all groups tested. The average of microtensile bond strength was decreased as the concentration and the application time of H2O2 were increased. Analysis showed significant correlation effect not only between the depth of the dentin and the concentration of H2O2 but also between the concentration of H2O2 and the application time(p < 0.05), while no significant difference existed among these three variables(p > 0.05). The higher H2O2 concentration, the more opened dentinal tubules under a scanning electron microscope(SEM) examination.

Keyword

Hydrogen peroxide; Microtensile bond strength; Deep dentin; Pulp chamber dentin; Oxidizing action; Free radical mechanism; Superbond C&B

MeSH Terms

Dental Pulp Cavity
Dentin
Electrons
Hydrogen
Hydrogen Peroxide
Tooth
Water
Hydrogen
Hydrogen Peroxide
Water

Figure

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  Figure 1 Schematic presentation of the disc preparation at the deep and the pulp chamber dentin

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  Figure 2 The manufacture of specimen

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  Figure 3 Schematic measurement of the microtensile bond strength

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  Figure 4 Control group(2K). Most surface was covered with smear layer and had scratches (black arrow) due to using a diamond bur. A. D1, B. D2, Bar=20µm.

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  Figure 5 With 20% H2O2 for 20min(2K). Dentinal tubules (white arrow head) were opened completely and the smear layer scarcely remained. A. D1, B. D2, Bar=20µm.

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  Figure 6 With 10% H2O2 for 20min(2K). Dentinal tubules (white arrow head) were opened in a part and the smear layer remained. A. D1, B. D2, Bar=20µm.

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  Figure 7 With 5% H2O2 for 20min(2K). A. D1, Dentinal tubules (white arrow head) were obscure and covered with the smear layer; B. D2, Dentinal tubules were clear and opened in a part. Bar=20µm.

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  Figure 8 With 2.5% H2O2 for 20min(2K). Dentinal tubules (white arrow head) were obscure and covered with the smear layer. A: D1; B: D2; the surface of D1 and D2 had a similar pattern; Bar=20µm.

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