J Gynecol Oncol.  2010 Sep;21(3):169-173. 10.3802/jgo.2010.21.3.169.

GST (GSTM1, GSTT1, and GSTP1) polymorphisms in the genetic susceptibility of Turkish patients to cervical cancer

• Kiran B
• Karkucak M
• Ozan H
• Yakut T
• Ozerkan K
• Sag S
• Ture M

• KMID: 1980853
• DOI: http://doi.org/10.3802/jgo.2010.21.3.169

Abstract

OBJECTIVE
This work investigates the role of glutathione S-transferase M1 (GSTM1), glutathione S-transferase T1 (GSTT1), and glutathione S-transferase P1 (GSTP1) enzymes and polymorphisms, which are found in phase II detoxification reactions in the development of cervical cancer.
METHODS
This study was conducted with 46 patients diagnosed with cervical cancer and 52 people with no cancer history. Multiplex PCR methods were used to evaluate the GSTM1 and GSTT1 gene polymorphism. However, the GSTP1 (Ile105Val) gene polymorphism was studied using a PCR-RFLP method. The patient and control groups were compared using a chi-square test with p<0.05.
RESULTS
In the patient group, statistical significance was determined for gravidity (p=0.03), parity (p=0.01), and the number of living children (p=0.01) compared to the control group. The gene frequency of GSTM1, GSTT1, and GSTP1 polymorphisms was evaluated. We observed that GSTM1 and GSTT1 null genotype frequencies were 54.3% and 32.6% respectively, while GSTP1 (Ile/Val), (Ile/Ile), (Val/Val) genotype frequencies were 52%, 44%, and 4%, respectively, in the cervical cancer patients. No statistical variation was determined between the control and patient groups in terms of GSTM1, GSTT1, and GSTP1 polymorphisms (p>0.05).
CONCLUSION
Our results demonstrate that GSTT1, GSTM1, and GSTP1 polymorphisms are not associated with cervical cancer in Turkish patients.

Keyword

Cervical cancer; GST gene; Polymorphism

MeSH Terms

Child
Female
Gene Frequency
Genetic Predisposition to Disease
Genotype
Glutathione Transferase
Gravidity
Humans
Metabolic Detoxication, Phase II
Multiplex Polymerase Chain Reaction
Parity
Uterine Cervical Neoplasms
Glutathione Transferase

Figure

• 

  Fig. 1 A representative multiplex PCR analysis of glutathione S-transferase (GST) polymorphisms. Albumin (350 bp), glutathione S-transferase T1 (GSTT1; 459 bp) and glutathione S-transferase M1 (GSTM1; 219 bp) genes PCR products resolved by agarose gel electrophoresis. Line MARKER is 100 bp DNA ladder. Lines 1 and 4 are both GSTT1 and GSTM1 present genotype, line 2 both GSTT1 and GSTM1 null genotype, line 3 is GSTT1 present genotype and GSTM1 null genotype.

• 

  Fig. 2 Photograph of the PCR products of the glutathione S-transferase P1 (GSTP1) gene after Alw 26 I enzyme cutting and on 3.5% agarose gel. Line MARKER shows the 100 bp DNA ladder, line 1 and 4 shows individuals with Ile/Ile genotype (176 bp), line 2 and 5 shows Ile/Val genotype (176 bp, 91 bp, 85 bp), and line 3 shows the Val/Val genotype (91 bp, 85 bp).

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