Korean J Pathol.  2011 Dec;45(6):604-611.

Foreign Body Reaction and Expression of Matrix Metalloproteinases/Tissue Inhibitor of Metalloproteinase by Injection of Mineral Fibers in Rats

• Seo DK
• Lee JI
• Kim JR

Abstract

BACKGROUND
The host response to natural fibers results in granuloma formation in an effort to limit tissue destruction. Matrix metalloproteinases (MMPs) are important molecules in the inflammatory granulomatous or reparative reaction. Here, we studied the foreign body reaction that occurs following natural fibers implantation by investigating MMPs and tissue Inhibitor of MMPs (TIMPs) in an in vivo model.
METHODS
Female Sprague-Dawley rats were treated with crocidolite fiber or fibrous talc via subcutaneous and intraperitoneal injections and immunohistochemistry was conducted to confirm the expression of MMPs and TIMP-2 in tissue sections.
RESULTS
We identified that mineral fibers elicited granulomas. Fibrous talc or intraperitoneal injection resulted in larger granulomas and severe tissue destruction compared with the lesions induced by crocidolite or subcutaneous injection. The expression of MMPs was elevated while granulomatous lesions were formed. The relative levels of MMPs were lower in the talc injected or intraperitoneal route models than those of crocidolite injected or subcutaneous injection models during the entire experiment.
CONCLUSIONS
These findings demonstrate that specific expression of MMPs/TIMP is inversely related to the grade of tissue destruction and suggest that expression of MMPs is required for promoting granuloma formation and limiting tissue destruction.

Keyword

Fibrous talc; Talc; Asbestos; Crocidolite; Foreign body reaction

MeSH Terms

Animals
Asbestos
Asbestos, Crocidolite
Female
Foreign Bodies
Foreign-Body Reaction
Granuloma
Humans
Immunohistochemistry
Injections, Intraperitoneal
Injections, Subcutaneous
Matrix Metalloproteinases
Mineral Fibers
Rats
Rats, Sprague-Dawley
Talc
Tissue Inhibitor of Metalloproteinase-2
Asbestos
Asbestos, Crocidolite
Matrix Metalloproteinases
Mineral Fibers
Talc
Tissue Inhibitor of Metalloproteinase-2