J Adv Prosthodont.  2015 Feb;7(1):21-26. 10.4047/jap.2015.7.1.21.

Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis

• Malkoc MA
• Demir N
• Sengun A
• Bozkurt SB
• Hakki SS

• KMID: 1974886
• DOI: http://doi.org/10.4047/jap.2015.7.1.21

Abstract

PURPOSE
To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).
MATERIALS AND METHODS
Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 microL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.
RESULTS
All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.
CONCLUSION
The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.

Keyword

Cytotoxicity; Temporary cements; Real-time cell analysis; Dental pulp-derived cells

MeSH Terms

Cell Survival
Microscopy, Electron, Scanning
Suspensions
Suspensions

Figure

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  Fig. 1 Dynamic monitoring of cultured dental pulp-derived cells (bDPCs) adhesion and cell proliferation.

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  Fig. 2 Light microscope images of cultured dental pulp-derived cells (bDPCs) at 30 and 120 minutes. Control, a: Rely X Temp E, b: Ultratemp, c: GC Fuji Temp, d: Rely X Temp NE.

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  Fig. 3 Scanning electron microscope images of cultured dental pulp-derived cells (bDPCs). a: Rely X Temp E, b: Ultratemp, c: GC Fuji Temp, d: Rely X Temp NE.

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