Int J Stem Cells.  2014 Nov;7(2):158-161. 10.15283/ijsc.2014.7.2.158.

Combining Autologous Peripheral Blood Mononuclear Cells with Fibroblast Growth Factor Therapy Along with Stringent Infection Control Leading to Successful Limb Salvage in Diabetic Patient with Chronic Renal Failure and Severe Toe Gangrene

• Osawa H
• Orii K
• Terunuma H
• Abraham SJ

• KMID: 1974604
• DOI: http://doi.org/10.15283/ijsc.2014.7.2.158

Abstract

Peripheral arterial disease (PAD) is a common complication of Diabetes Mellitus (DM) and often culminates in amputation of the affected foot. Pseudomonas aeruginosa infections associated with PAD are difficult to treat due to their multi-drug resistance. Herein we report a 38 year old male who reported with DM, chronic kidney disease (CKD) and rest pain of the right second toe in October 2011. He underwent percutaneous transluminal angioplasty (PTA) which was unsuccessful. The gangrene of the toes worsened and amputation of the right second toe was done. Bacteriological examination showed presence of P. aeruginosa which during the course of antibiotic therapy became multi-drug resistant. Gangrene and abscess of the foot worsened and amputation of the right third toe was performed. Then autologous peripheral blood mononuclear cell (PBMNC) therapy was performed but as infection control could not still be achieved, the fourth toe was amputated. A protocol of foot bath using carbonic water, local usage of antibiotics (Polymyxin-B), and basic fibroblast growth factor (b-FGF) spray was then employed after which the infection could be controlled and improvement in vascularity of the right foot could be observed in angiography. This combined approach after proper validation could be considered for similar cases.

Keyword

Diabetes mellitus (DM); peripheral arterial disease (PAD); Pseudomonas aeruginosa; Cell therapy; Infection control

MeSH Terms

Abscess
Amputation
Angiography
Angioplasty
Anti-Bacterial Agents
Baths
Carbon
Cell- and Tissue-Based Therapy
Diabetes Mellitus
Drug Resistance, Multiple
Fibroblast Growth Factor 2
Fibroblast Growth Factors*
Foot
Gangrene*
Humans
Infection Control*
Kidney Failure, Chronic*
Limb Salvage*
Male
Peripheral Arterial Disease
Pseudomonas aeruginosa
Renal Insufficiency, Chronic
Toes*
Water
Anti-Bacterial Agents
Carbon
Fibroblast Growth Factor 2
Fibroblast Growth Factors
Water

Figure

• 

  Fig. 1. (A) Gangrene of right 2nd toe. (B) Amputation of the right 2nd toe. (C) Amputation of the right 3rd toe and wound debridement. (D) Improvement in granulation and infection control. (E) Right foot at the time of discharge showing successful limb salvage.

• 

  Fig. 2. Digital subtraction angiography (DSA) images of the right limb. (A) At the time of admission showing the occlusion of the anterior and posterior tibial arteries. (B) After the combined therapy protocol showing worsening of occlusion in the main route of posterior tibial artery but development of the collateral arteries.

Reference

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