Genomics Inform.  2003 Sep;1(1):50-54.

Genome Mapping of an Extreme Thermophile, Thermus caldophilus GK24

• Park JH
• Park BC
• Koch SH
• Kim JS
• Koh JH
• Yang MH
• Kim YS
• Kim CH
• Kim MH
• Kwon ST
• Lee DS

Abstract

Genome of an extreme thermophile, Thermus caldophilus GK24 has been analyzed to construct the genomic map. The genomic DNAs encapsulated in agarose gel were digested with SspI, EcoRI, SpeI, and HpaI restriction endonucleases, and then the resulting genomic DNA fragments were analyzed by pulsed-field gel electrophoresis. Its restriction map has been constructed by analyzing sizes of the restriction fragments obtained from both complete and partial digestions. The circular form of its genome was composed of about 1.98 Mbp and a megaplasmid. The genomic loci for the genes of xylose isomerase, thioredoxin, tRNA-16S rRNA, 23S rRNA, L5 ribosomal protein, ADP-glucose pyrophosphorylase, DNA-ligase, and Tca DNA polymerase were determined by both Southern hybridization and PCR.

Keyword

PFGE; genome map; Thermus caldophilus GK24

MeSH Terms

Chromosome Mapping*
DNA
DNA Restriction Enzymes
Electrophoresis, Gel, Pulsed-Field
Genome*
Glucose-1-Phosphate Adenylyltransferase
Polymerase Chain Reaction
Ribosomal Proteins
Sepharose
Thermus*
Thioredoxins
Xylose
DNA
DNA Restriction Enzymes
Glucose-1-Phosphate Adenylyltransferase
Ribosomal Proteins
Sepharose
Thioredoxins
Xylose