J Clin Pathol Qual Control.  2001 Jun;23(1):185-191.

The Analysis of Engraftment Results of Autologous Peripheral Blood Stem Cell Transplantation in Kosin Medical Center in 1999

• Hwang HY
• Kim MH
• Kim YS
• Eo WK
• Park JS
• Jeong SH
• Lee CS

Abstract

BACKGROUND: Most transplant groups have established their own guideline to monitor the timing of successful leukapheresis and minimal number of CD34+ cells to ensure engraftment in autologous peripheral blood stem cell transplantation. We therefore retrospectively analyzed values that could indicate collection and engraftment of autologous peripheral blood stem cell, and intended to establish our own criteria for successful autologous peripheral blood stem cell transplantation.
METHODS
We had investigated counts for CD34+ cell, mononuclear cells and leukocytes in 52 peripheral blood samples, together with their corresponding leukapheresis components, to measure the best threshold of CD34+ cells for successful harvesting and engraftment of autologous peripheral blood stem cell transplantation. In addition, the predictive values of this measure have been compared with those of other commonly used criteria, such as the WBC count and mononuclear cell count in peripheral blood.
RESULTS
A good correlation between the number of CD34+ cells per microliter in peripheral blood and of CD34+ cells per kg collected was found at each apheresis(r=0.69, P=0.00). Moreover, the presence of > 5 CD34+ cells per microliter in peripheral blood ensured a good yield from the harvest in all patients (P=0.00), and the number of circulating CD34+ cells more than 20 per microliter ensured 2.9x10(6) CD34+ cells per kg in harvesting products. All patients receiving > or = 1.1x10(6) CD34+ cells per kg achieved engraftment within a reasonable number of days(>0.5x10(9)/microliter neutrophils by mean Day 10 and>20x10(9)/microliter platelets by mean Day 11).
CONCLUSIONS
This report showed that the number of circulating CD34+ cells was a good marker to monitor the timing of leukapheresis in most cases, and a minimum of more than 5 CD34+ cells per microliter in peripheral blood was required to initiate leukapheresis. The dose of more than 1.1x10(6) CD34+ cells per kg was needed to guarantee post-transplant hematopoietic recovery, however, this needs to be confirmed by further evaluation because of small numbers of analyzed cases.

MeSH Terms

Cell Count
Humans
Leukapheresis
Leukocytes
Neutrophils
Peripheral Blood Stem Cell Transplantation*
Retrospective Studies
Stem Cells