J Korean Assoc Maxillofac Plast Reconstr Surg.  2006 Mar;28(2):127-135.

Hsp27 modulation of implant-associated metal ion cytotoxicity of osteoblastic cells

• Yoon JH
• Ha DJ
• Rim JS
• Kwon JJ
• Jang HS
• Lee ES
• Kim DS

Abstract

OBJECTIVES
The extent of bone formation that occurs at the interface of metallic implants and bone is determined by the number and activity of osteoblastic cells. Stress proteins may be contributing determinants of cell viability in altered environments. Hsp27 is a small Mr hsp which is known as a molecular chaperone. METHODS: To better understand how heat shock protein 27 contributes to endosseous implant - associated metal ions affects on osteoblastic cell viability, the effect of chromium and titanium ions were compared to effects of cadmium ions in the ROS17/2.8 osteoblastic cell line. RESULTS: ROS17/2.8 osteoblastic cell line demonstrated ion - specific reductions in growth; reductions were significantly greater for cadmium than for chromium or titanium. Chromium impaired growth of cultures without altering cell viability measured using the MTT assay. A stable transformed cell line expressing additional hsp27(clone "A7") was resistant to the toxic effects of titanium and partially protected from cadmium toxicity. CONCLUSIONS: A role for hsp27 in protection of osteoblastic cells from metal ion toxicity is supported by the chromium - induced elevations in hsp27 abundance and the behavior of the A7 cell line in response to metal ions in culture. Similar biochemical responses to altered cellular environments may contribute to the fate of tissues adjacent to select metallic implants.

Keyword

Metallic implants; Heat shock protein 27; Chromium; Titanium; Cadmium

MeSH Terms

Cadmium
Cell Line
Cell Line, Transformed
Cell Survival
Chromium
Heat-Shock Proteins
HSP27 Heat-Shock Proteins
Ions
Molecular Chaperones
Osteoblasts*
Osteogenesis
Titanium
Cadmium
Chromium
HSP27 Heat-Shock Proteins
Heat-Shock Proteins
Ions
Molecular Chaperones
Titanium