J Korean Neurosurg Soc.  1995 Mar;24(3):253-261.

Distrubution of gamma-Glutamyltransferase in Blood-Brain Barrier Pericytes Using Monoclonal Anti gamma-Glutamyltransferase Antibodies

• Lee BK
• Kim MK
• Shin KM
• Ryu CK

Abstract

gamma-Glutamyltransferase(GGT: E.C. 2.3.2.2.) is a glycoprotein enzyme which is involved in glutathione metabolism and amino acid transport through the plasma membrane. It is distributed widely in several organs including liver, kidney, pancrease and brain. GGTs derived from the brain of Wister rats and BALB/c mice were biochemically purified to a specific activity of 4246.2, 862.1 units per mg of protein, a purification folds 93.7, 43.8 and the final yield 65.8, 44.0% respectively. Electrophoretic pattern of purified GGTs from rats and mice brain shows very similar protein fraction each other. We have produced six monoclonal antibodies(GGT-Mab 1-6) against 2-acetamidogluorene treated rat liver GGT. Using these GGT-Mab 1-6 we performed immunohistochemistry(IHC) to study the distribution of GGT isozymes in normal tissues of rat brain and in neoplastic tissues of human brain. The results indicated that human brain GGT was localized in pericytes of blood-brain barrier, especially in the blood-rich portion of the brain(e.g. cerebellum of rat, meningioma and craniopharyngioma of human). Therefore these Maps may be used to evaluate the distribution of GGT isozymes in different tissues.

Keyword

gamma-Glutamyltransferase; Monoclonal antibodies; Blood-brain barrier

MeSH Terms

Animals
Antibodies*
Antibodies, Monoclonal
Blood-Brain Barrier*
Brain
Cell Membrane
Cerebellum
Craniopharyngioma
gamma-Glutamyltransferase*
Glutathione
Glycoproteins
Humans
Isoenzymes
Kidney
Liver
Meningioma
Metabolism
Mice
Pancreas
Pancrelipase
Pericytes*
Rats
Antibodies
Antibodies, Monoclonal
Glutathione
Glycoproteins
Isoenzymes
Pancrelipase
gamma-Glutamyltransferase