The Effect of Poly-L-lysine on Proliferation and Differentiation of Preadipocyte in the Alginate Gels

Kwon, Ho; Song, Jin Kyung; Rhie, Jong Won; Cho, Yong Nam; Lim, Jin Soo; Chung, Sang Hoon; Ahn, Sang Tae

Abstract

Alginate is widely used for scaffold in tissue engineering. However, it has a limitation of cell proliferation due to the lack of cell-to-matrix adhesion. Authors were trying to find out that the alginate gel become an efficient three-dimensional biomatrix in case of mixing with poly-L-lysine (PLL). After harvesting preadipocyte from rat epididymal fat, the proper concentration of PLL for an efficient cell culture was examined in the alginate gel and the level of proliferation of cells were measured in order to find out the efficacy of PLL for the experimental group(alginate/PLL mixed gel) compared to the control group(alginate gel without PLL). In addition, the number of surviving cell was counted and the fat cell stained with oil-red O was observed on the 21st day of the culture. The preadipocytes in the alginate gel were most viable in the PLL concentration of 50 microgram/ml. After 4 days in culture, the level of cell proliferation and the number of preadipocytes were significantly higher in the experimental group than those in the control group. A small number of fat cells stained with oil-red O were starting to be appeared on the 14th day and the larger number of cells on the 21st day of the culture in two groups. These results suggest that PLL increased the proliferation of preadipocyte in the alginate gel through the enhancement of cell-to-matrix adhesion. It also shows that alginate has the advantage of inducing the differentiation of preadipocyte in case of alginate/PLL mixed gel. In conclusion, alginate/PLL mixed gel is turned out to be effective for making a three-dimensional biomatrix.