Korean J Anat.  2006 Apr;39(2):81-90.

Effect about Neurite Extension of FS390, an Inhibitor of Exocytosis in Rat Hippocampal Neurons and PC12 Cells

• Choi MK
• Chon GH
• Lee YS

Abstract

FS390, a novel microbial metabolite from Streptomyces spp. was identified as a small molecular substance and shown a inhibition activities for the release of neurotransmitter from rat hippocampal neuron and PC12 cells. FS390 is an inhibitor of trifiated norepinephrine ([3H]-NE) release in high K+ buffer solution containing ionomycin, indicating that FS390 inhibits neurotransmitter release after the influx of Ca2+ ions. When examined the effect of FS390 on beta-glucuronidase release from guinea pig neurophils, FS390 inhibited beta-glucuronidas release: when treated with 5 microgram/mL of FS390, which was not induced cellular cytotoxicity. The fact that the beta-glucuronidase release in neutrophil and norepinephrine release in neuron was inhibited suggests the similarity in the locations and the mechanisms of FS390 action targets. When treated with 5 microgram/mL of FS390, [3H]-NE release and neurite extension for both rat hippocampal neurons and PC12 cells were prevented. These observations of FS390 functioning as an inhibitor of neurotransmitter release suggest that FS390 has an important role in synaptic transmission in neuron.

Keyword

Neuron; Exocytosis; Inhibitor; Neurotransmitter; Neurite

MeSH Terms

Animals
Exocytosis*
Glucuronidase
Guinea Pigs
Ionomycin
Ions
Neurites*
Neurons*
Neurotransmitter Agents
Neutrophils
Norepinephrine
PC12 Cells*
Rats*
Streptomyces
Synaptic Transmission
Glucuronidase
Ionomycin
Ions
Neurotransmitter Agents
Norepinephrine