Int J Oral Biol.  2016 Dec;41(4):231-236. 10.11620/IJOB.2016.41.4.231.

Reactive Oxygen Species are Involved in Y-27632-induced Neurite Outgrowth in PC12 Cells

• Park SY
• Moon SA
• An JM
• Kim DS
• Seo JT

• KMID: 2368587
• DOI: http://doi.org/10.11620/IJOB.2016.41.4.231

Abstract

Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.

Keyword

Y-27632; ROCK; PC12 cells; neurite outgrowth

MeSH Terms

Acetylcysteine
Animals
Neurites*
Neurons
PC12 Cells*
Phosphotransferases
Reactive Oxygen Species*
Acetylcysteine
Phosphotransferases
Reactive Oxygen Species