Korean J Fertil Steril.  1998 Dec;25(3):331-340.

Activation of Porcine Oocytes Following Intracytoplasmic Injection of Various Sperm Components and foreign species spermatozoa

• Jun SH
• Shin JS
• Do JT
• Kwon JK
• Kim NH
• Lee HT
• Chung KS

Abstract

SUMMARY: We determined the incidence of activation, male pronuclear formation and apposition of pronuclei in porcine oocytes following intracytoplasmic injection of various porcine sperm components and foreign species spermatozoa, such as mouse, human or cattle. The porcine oocytes were activated by injection of a spermatozoon or an isolated sperm head. Neither isolated sperm tail nor perinuclear material removed sperm head activated oocytes. Because injection of mouse, bovine or human spermatozoon activated porcine oocytes, the sperm born activation factors is not strict species specific. Male pronuclear formation and pronuclear apposition were observed in the porcine oocytes following injection of porcine, bovine, mouse or human spermatozoa. The electrical stimulation following sperm cell injection did not enhance the incidence of male pronuclear formation nor pronuclear apposition compared with sperm cell injection alone (p>0.1). Mitosis and two cell division in some oocytes were observed at 20 to 24 h after injection of porcine spermatozoon. However, none of oocytes following injection of mouse, bovine or human spermatozoa developed to the mitotic metaphase or normally divided to the two cell stage. These results suggested that the oocyte activating factor(s) presented in the perinuclear material and it is not species specific for the porcine oocyte.

MeSH Terms

Animals
Cattle
Cell Division
Electric Stimulation
Humans
Incidence
Male
Metaphase
Mice
Mitosis
Oocytes*
Sperm Head
Sperm Tail
Spermatozoa*