Korean J Thorac Cardiovasc Surg.  2000 Jan;33(1):60-67.

Microsatellite Instability and p53 , k-ras c-myc Oncoprotein Expression in Non-Small Cell Lung Carcinoma

• Rha SJ
• Kwak MS

Abstract

BACKGROUND: Microsatellites are short-tandem repeated uncleotide sequences present throughout the human genome. Alterations of microsatellites have been termed microsatellite instability(MI). It has been generally known that microsatellite instability detected in hereditary non-polyposis colorectal cancer (HNPCC) reflects genetic instability that is caused by impairments of DNA mismatch repair system regarding as a novel tumorigenic mechanism. A number of studies reported that MI occurred at varying frequencies in non-small cell lung carcinoma (NSCLC). However It has been unproven whether MI could be a useful market of genetic instability and have a clinical significance in NSCLC. MATERIAL AND METHOD: We have examined whether MI can be observed in thirty NCSLC using polymerase chain reaction whether such alterations are associated with other molecular changes such as p53, K-ras and c-myc oncoproteins expression detected by immunohistochemical stain,. RESULT: MI(+) was observed in 16.6%(5/30) and MI(-) was 83.3% (25/30) Average age was 50+/-7.5 year-old in MI(+) group and 57+/-6.6 year-old in MI(-) group. Two year survival rate in MI(=) group (20% 1/5) was worse than MI(-) group (64% 16/25) with a statistic difference. (P=0.04) The positive rate of K-ras oncoprotein expression and simultaneous expression of 2 or 3 oncoproteins expression were higher in MI(+) group than MI(-) group with a statistic difference(P=0.05, P=0.01)
CONCLUSIONS
From, these results the authors can conclude that MI is found in some NSCLC and it may be a novel tumorigenic mechanism in some NSCLC. We also conclude that MI could be used as another poor prognostic factor in NSCLS.

Keyword

Carcinoma, non-small cell, lung; Neoplasm manker; Lung neoplasm

MeSH Terms

Colorectal Neoplasms
DNA Mismatch Repair
Genome, Human
Humans
Lung Neoplasms
Lung*
Microsatellite Instability*
Microsatellite Repeats*
Oncogene Proteins
Polymerase Chain Reaction
Survival Rate
Oncogene Proteins