Comparison Between the Costal Cartilage Mounted Autoloous Pericardial Patch and t he Bovine Pericardial Patch as a Bronchial Substitute for Bronchial Reconstruction in Dogs

Lee, Hae Young; Park, Chan Beom; Cho, Kuhn Hyun

Abstract

BACKGROUND: Various bronchoplastic procedures have been attempted in patients with long segmental bronchial stenosis, but it is still not clear which procedure is the best method for restoring bronchial patency. MATERIAL AND METHOD: To study the feasibility of designed patch as a bronchial substitute in bronchoplastic procedure, 10 experimental dogs of each 5 were divided into two groups according to the different patches applied. In group A (n=5), 0.5X0.5 cm sized rectangular defects were created on ventral aspect of left main bronchus and were then covered with patches made of costal cartilage mounted autologous pericardium. In group B(n=5), same sized bronchial defects were created and covered by patches made by glutaraldehyde treated bovine pericardium. Dogs were sacrificed on the twelfth weeks after bronchoplasty and the macroscopic and microscopic findings were examined. RESULT: In group A, external surface of grafted patches revealed some membranous adhesions with surrounding connective tissue and the margin of patches were smoothly incorporated into native bronchial wall. The bronchial lumen kept good integrity without stenosis and preserved the glistening mucosa well. Formation of new transitional epithelial bridging between native bronchial wall and grafted patches with tiny scattered granulation tissues were present. In group B, external surface of grafted patches were brownish shrunken with partial collapse of bronchial lumen. Inner surface showed dirty granulation at the edge of native bronchus and grafted patches with intermingled hemorrhages. New epithelial growth into the patches had not occurred.
CONCLUSION
These results suggest that costal cartilage mounted autologous pericardial patches could be considered as one of the appropriate bronchial substitutes for covering bronchial defects since it maintains bronchial stability and forms epithelial bridging with native bronchus.