Korean J Clin Microbiol.  1999 Sep;2(2):135-143.

Microplate Identification System of Enterobacteriaceae

Abstract

BACKGROUND: To access the accuracy and clinical usefulness of microplate identification (ID) system for the identification of Enterobacteriaceae, we compared microplate ID system with API 20E(bioMerieux, Etoile, France).
METHODS
Ninety-two cultures of Enterobacteriaceae and one isolate of Aeromonas species were simultaneously identified by microplate ID system and the API 20E. Twenty biochemical tests used in microplate ID system were lactose, sucrose, and H2S in Kligler's iron agar media; indole, sucrose, raffinose, arabinose, trehalose, adonitol, dulcitol, sorbitol, cellibiose, methy-red, phenylalanine deaminase, ornithine decarboxylase, lysine decarboxylase, arginine dihydrolase, urease, and citrate in microplate; and oxidase test. The identification was obtained by considering percent likelihood(% ID), modal frequency and ID score method.
RESULTS
Among the 92 cultures of Enterobacteriaceae and one isolate of Aeromonas species, agreement rate of identification according to the % ID between microplate ID system and API 20E were 90.3% to the species level and 97.8% to the genus level.
CONCLUSIONS
For the identification of clinical Enterobacteriaceae isolates, the microplate ID system compares favorably with API 20E in identification accuracy and have the advantage of costsaving and easy to use.

Keyword

Enterobacteriaceae; Aeromonas; Microplate identification system; API 20E

MeSH Terms

Aeromonas
Agar
Arabinose
Arginine
Citric Acid
Enterobacteriaceae*
Galactitol
Iron
Lactose
Lysine
Ornithine Decarboxylase
Oxidoreductases
Phenylalanine
Raffinose
Ribitol
Sorbitol
Sucrose
Trehalose
Urease
Agar
Arabinose
Arginine
Citric Acid
Galactitol
Iron
Lactose
Lysine
Ornithine Decarboxylase
Oxidoreductases
Phenylalanine
Raffinose
Ribitol
Sorbitol
Sucrose
Trehalose
Urease
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