Evaluation of V Form Microplate Method for Irregular Antibody Screening of Blood Donors

Lee, Chung Young; Han, Sang Moo; Lee, Won Nam

Abstract

BACKGROUND
Clinically significant hemolytic transfusion reaction by warm-reacting irregular antibodies are tested up to antiglobulin phase. It is known that microplate method show similar or better than conventional tube method. Authors examined the irregular antibody screening of blood donors using V form microplate method at Korean Red Cross Blood Centers and evaluated its usefulness. METHOD: Total 200,246 sera from blood donors were performed antibody screening using the automated blood testing system IBG (IBG Co, UK) and V form microplate method. For the identification in antibody screening positive sera, two kinds of panel cell (Search cyte I,II, Data cyte, DADE Co, USA, and Central Red Cross Blood Center, Korea) were used. RESLUTS: Irregular antibodies were screened in 1,581 cases out of 200,246 samples (positive rate;0.79%). However, the actually identified antibodies were 1,086 (0.54%). The identified antibodies were mostly cold antibody anti-Lea (681 cases), anti-Leb (271 cases), anti-P1 (53 cases) and anti-S, anti-Lua, anti-Fya detected only one case, respectively. The incidence of warm antibody was very low. For antibody screening, the reactivity of V form microplate was much higher than IBG system.
CONCLUSION
It would be useful to apply V form microplate method for large number of donor screening. An advantage of this method is its possible automation with substantial time and cost effectiveness. Consequently, the adaptation of this method for antibody screening is suitable for Korean Red Cross Blood Centers.