Change of Cellular Immunity and T Cell Subset of Mouse Immunocytes Activated by Fractionated Bacillus Calmette-Guerin

Lee, Eunsik; Jeong, Hyeon; Yoon, Sang Jin; Lee, Hae Won; Min, Kyung Joon; Choi, Yong Kyung; Lee, Chongwook

Abstract

PURPOSE: To develope the bacillus Calmette-Guerin(BCG) antitumor component therapy which could reduce the adverse effect of intravesical BCG therapy, we investigated the effect of the fractions of BCG on the immune responses of mouse and compared with whole BCG.
MATERIALS AND METHODS
Seven fractions, 4(fraction 1, 2, 3, 4) from BCG cell wall and 1 (fraction 7) front BCG cytoplasm and 2(fraction 5, 6) mixed, were collected by multiple ultrasonification and centrifugation of BCG solution and the difference of each fraction was confirmed by spectrophotometric absorbance. Change of lymphokine killer(LAK), natural killer(NK) and tumor cell dependent cytotoxic(TCDC) activities of mouse spleen cells were investigated by 51Cr releasing assay and proportion of T cell subset in mouse spleen and peritoneal lymhocytes were investigated by flow cytometric analyses using monoclonal antibodies to mouse CD4, CD8 and CD2S cells.
RESULTS
In LAK and NK activities, fraction 1, 2 and 3 showed similar results with whole BCG. In TCDC activity, the all 7 fraction showed lower activities compared with BCG. In T cell subset analyses, similar results were found in fraction 1, 2 and 3 compared with whole BCG.
CONCLUSIONS
We could suggest that the antitumor activity of BCG seems to be mediated by some component in cell wall. Further investigations which could elucidate these findings should be necessary.