Tuberc Respir Dis.  2000 Feb;48(2):223-235. 10.4046/trd.2000.48.2.223.

Perfluorocarbon Does Not Inhibit Chemokine Expression in Airway Epithelial Cells

Affiliations
  • 1Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, Seoul, Korea.

Abstract

BACKGROUND: Liquid ventilation is associated with decreased inflammatory response in an injured lung. This study was performed to investigate if whether perfluorocarbon (PFC) can decrease chemokine expression in airway epithelial cells. METHODS : A549 cells were used for airway epithelial cells and perfluorodecalin for PFC. To expose cells to PFC, lower chamber of Transwell a plate was used. This study was performed in two parts. In the first part, we examined whether PFC could decrease chemokine expression in airway epithelial cells through inhibition of other inflammatory cells. Peripheral blood mononuclear cells (PBMC's) were isolated and stimulated with lipopolysaccharide (LPS, 10 mg/mL) for 24 hours with or without exposure to PFC. Then A549 cells were stimulated with conditioned media (CM) containing the culture supernatants of PBMC . After 24 hours, the expressions of interleukin-8 (IL-8) and RANTES were measured. In the second part of the study, we studied whether PFC could directly suppress chemokine expression in airway epithelial cells. A549 cells were stimulated for 24 hours with interleukin-1b and/or tumor necrosis factor-a with or without exposure to PFC (,)and then the chemokine expression was measured. Northern analysis was used to measure the mRNA expression (,) and ELISA was used for immunoreactive protein measurements in culture supernatant. RESULTS: 1. IL-8 and RANTES mRNA expression and immunoreactive protein production were increased significantly by CM from LPS-stimulated PBMC in A459 cells compared with CM from unstimulated PBCM(p<0.05) (,)but exposure of PFC had no significant effect on either mRNA expression immunoreactive protein expression. 2. IL-8 and RANTES mRNA expression and immunoreactive protein production were increased significantly by IL-1b and TNF-a in A549 cells(p<0.05)(,)but exposure of PFC had no significant effect on either mRNA expression or immunoreactive protein production.
CONCLUSION
Decreased chemokine expression of airway epithelial cells may not be involved in decreased inflammatory response observed in liquid ventilation. Further studies on possible mechanisms of decreased inflammatory response are warranted.

Keyword

Fluorocarbon; Cytokine; Liquid ventilation; Inflammation

MeSH Terms

Chemokine CCL5
Culture Media, Conditioned
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Inflammation
Interleukin-8
Liquid Ventilation
Lung
Necrosis
RNA, Messenger
Chemokine CCL5
Culture Media, Conditioned
Interleukin-8
RNA, Messenger
Full Text Links
  • TRD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr