Korean J Fertil Steril.  2006 Jun;33(2):115-123.

Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone)

Affiliations
  • 1Department of Clinical Pathology, Yonsei University, Seoul, Korea.
  • 2Department of Molecular Science and Technology, Ajou University, Suwon, Korea. parker@ajou.ac.kr

Abstract


OBJECTIVE
To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. METHOD: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins.
RESULTS
GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin beta3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II.
CONCLUSION
GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.

Keyword

GnRH; Integrin; FAK; ERK; Phosphorylation

MeSH Terms

Cell Proliferation*
Endometrial Neoplasms*
Female
Gonadotropin-Releasing Hormone*
Humans
Immunoblotting
Integrin beta3
Phosphorylation
Signal Transduction
Thymidine
Gonadotropin-Releasing Hormone
Integrin beta3
Thymidine
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