Gut Liver.  2013 Jul;7(4):430-436.

Investigation of Interleukin-10 Promoter Polymorphisms and Interleukin-10 Levels in Children with Irritable Bowel Syndrome

• Hua MC
• Chao HC
• Yao TC
• Lai MW
• Huang JL
• The PATCH Study Group

Abstract

BACKGROUND/AIMS
The aim of this study was to investigate whether genetic variations at positions -1082, -819, and -592 in the interleukin (IL)-10 promoter affect IL-10 production in children with irritable bowel syndrome (IBS).
METHODS
Ninety-four children with IBS and 102 children as healthy controls (HCs) were enrolled. Genomic DNA was extracted, and IL-10 -1082, -819, and -592 polymorphisms were detected by direct sequencing from all participants. Peripheral blood mononuclear cells (PBMCs) from 46 IBS children and 38 HCs were isolated and cultured with and without 5 ng/mL Escherichia coli lipopolysaccharide (LPS). IL-10 levels in the culture supernatants were measured by enzyme-linked immunosorbent assay.
RESULTS
There were no significant differences in the distribution of IL-10 -1082, -819, and -592 polymorphisms or in the allele and haplotype frequencies between IBS children and HCs. PBMCs from children with IBS had significantly lower IL-10 levels after LPS stimulation than PBMCs from HCs (p=0.011); however, LPS-induced IL-10 levels in PBMCs with different genotypes of -819 and -592 polymorphisms were not significantly different between IBS patients and HCs.
CONCLUSIONS
Although significantly lower LPS-induced IL-10 production by PBMCs was noted, it is unlikely that IL-10 production was fully genetically determined in our IBS children. ClinicalTrials.gov identifier: NCT01131442.

Keyword

Irritable bowel syndrome; Child; Interleukin-10; Interleukin-10 gene polymorphisms

MeSH Terms

Alleles
Child
DNA
Escherichia coli
Genetic Variation
Genotype
Haplotypes
Humans
Interleukin-10
Interleukins
Irritable Bowel Syndrome
DNA
Interleukin-10
Interleukins