Cancer Res Treat.  2005 Aug;37(4):228-232.

Expression of Maspin is associated with the Intestinal Type of Gastric Adenocarcinoma

Affiliations
  • 1Laboratory of Gastroenterology and Hepatology, Hallym University Medical Center, Seoul, Korea.
  • 2Department of Pathology, Hallym University Medical Center, Seoul, Korea. esnam2003@yahoo.co.kr
  • 3Department of Internal Medicine, Hallym University Medical Center, Seoul, Korea.

Abstract

PURPOSE
Maspin is known as a tumor suppressor gene, but its significance has been questioned in various human cancers. The aim of this study was to investigate the expression pattern of Maspin in human gastric adenocarcinomas and its possible correlation with clinicopathological findings. MATERIALS AND METHODS: The expression of Maspin mRNA was measured by nested RT-PCR using 60 frozen adenocarcinomas of the stomach and 31 noncancerous tissues from the proximal resection margin. Immunohistochemical study for Maspin protein expression was carried out using 62 paraffin-embedded tissues, composed of both cancer and noncancerous tissues. RESULTS: Maspin mRNA expression was detected in 80.0% (48 of 60) of the gastric adenocarcinomas, but in only 22.6% (7 of 31) of the normal gastric mucosa (p<0.001). The positive rate of Maspin protein expression was higher in the adenocarcinomas than the normal tissues (62.9% vs. 27.4%, p<0.05). In addition, the intestinal type of tumors showed significantly higher expression levels compared to the diffuse type of tumors (81.5% vs. 48.6%, p<0.05). CONCLUSION: Our results suggest that Maspin is frequently expressed in human gastric cancers, and its expression might be associated with tumorigenesis of the intestinal type of gastric cancer.

Keyword

Maspin; Gastric adenocarcinoma; Nested RT-PCR; Immunohistochemistry

MeSH Terms

Adenocarcinoma*
Carcinogenesis
Gastric Mucosa
Genes, Tumor Suppressor
Humans
Immunohistochemistry
RNA, Messenger
Stomach
Stomach Neoplasms
RNA, Messenger
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