J Korean Assoc Oral Maxillofac Surg.  2015 Jun;41(3):119-123. 10.5125/jkaoms.2015.41.3.119.

Effects of fresh mineralized dentin and cementum on socket healing: a preliminary study in dogs

• Kadkhodazadeh M
• Ghasemianpour M
• Soltanian
• Sultanian
• Ahmadpour
• Amid R

• KMID: 1797840
• DOI: http://doi.org/10.5125/jkaoms.2015.41.3.119

Abstract

OBJECTIVES
Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative material for alveolar healing.
MATERIALS AND METHODS
This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premolars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cutters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to 500 microm. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as mean+/-standard deviation and compared with t-test results.
RESULTS
The ratio of SA(bone) to total area of each probe was determined and was 170+/-16 microm2 for the control group and 71+/-14 microm2 for the DC group, a significant difference (P<0.05).
CONCLUSION
DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.

Keyword

Dentin; Cementum; Tooth extraction; Healing

MeSH Terms

Animals
Bicuspid
Biopsy
Bone Regeneration
Crowns
Dental Cementum*
Dentin*
Dogs*
Elevators and Escalators
Intercellular Signaling Peptides and Proteins
Minerals
Surgical Instruments
Tooth
Tooth Extraction
Transplants
Intercellular Signaling Peptides and Proteins
Minerals

Figure

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  Fig. 1 A stereological counting frame was superimposed on each randomized field. This frame is composed of 30 squares (each square, 25 µm). ×40.

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  Fig. 2 Dentin/cementum particle (asterisk) and a blood vessel (arrow) in an alveolar socket at the end of the second week. H&E staining, ×40.

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  Fig. 3 New bone formation (arrowhead), particles of dentin/cementum (arrow) in the periphery, and newly formed bone adjacent to the particles at the end of the eighth week. H&E staining, ×20.

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  Fig. 4 Bone formed in a centripetal pattern. Bone spicules (arrowhead), connective tissue (asterisk) and a blood vessel (arrow) in the control group at the eighth week. H&E staining, ×20.

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  Fig. 5 Red blood cells, venules (arrowhead), and connective tissue (asterisk) in the control group at the eighth week. H&E staining, ×20.

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