J Korean Med Sci.  2004 Jun;19(3):426-430. 10.3346/jkms.2004.19.3.426.

Molecular Analysis of HLA Class ll-Associated Susceptibility to Neuroinflammatory Diseases in Korean Children

• Oh HH
• Kwon SH
• Kim CW
• Choe BH
• Ko CW
• Jung HD
• Suh JS
• Lee JH

• KMID: 1786823
• DOI: http://doi.org/10.3346/jkms.2004.19.3.426

Abstract

The work was done to study immunogenetic peculiarities of neuroinflammatory diseases among Korean children. A total of 13 children with neuroinflammatory diseases (8 males and 5 females; mean age 4.6+/-2.6 yr) were consecutively recruited. Genomic typing was performed on their HLA DRB/HLA DQB genes using PCR-SSOP/ SSP techniques with gel immunoelectrophoresis. The frequencies of HLA-DR1* 15 in children with acute disseminated encephalomyelitis (ADEM) (31%) and DQB1* 06 in other neuroinflammatory diseases (38%) were significantly increased compared with control subjects. The frequencies of HLA-DRB3*0202 (100%), HLA-DRB1*1302 (67%), HLA-DRB3*0301 (67%), and HLA-DQB1*0301 (67%) were significantly increased in children with multiple sclerosis and the frequencies of HLA-DRB1*1501 (40%) and HLA-DRB5*0101 (40%) were significantly increased in children with ADEM. HLA-DRB1*1401, HLA- DRB3*0202, and HLA-DQB1*0502 were found in children with acute necrotizing encephalopathy. In conclusion, HLA-DR1*15 and DQB1*06 may be involved in susceptibility to inflammation in Korean children. The frequencies of HLA-DRB1*1501, HLA-DRB5*0101, HLA-DRB3*0301, and HLADQB1* 0602 were not as high in Korean children with multiple sclerosis as in western children. However, HLA-DRB3*0202 was seen in all children with multiple sclerosis. Our data may provide further evidence that the immunogenetic background of neuroinflammatory diseases in Korean is distinctly different from the ones in western countries. Further studies are necessary to confirm this finding.

Keyword

Neuroinflammatory Diseases; HLA-DR Antigens; HLA-DQ Antigens; Encephalomyelitis, Acute Disseminated; Multiple Sclerosis; Leucoencephalitis, Acute Hemorrhagic

MeSH Terms

Alleles
Child
Child, Preschool
Electrophoresis
Encephalomyelitis/genetics
Female
Genes, MHC Class II/*genetics
*Genetic Predisposition to Disease
Genotype
Human
Inflammation/*genetics
Male
Multiple Sclerosis/genetics
Neurons/*pathology
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Support, Non-U.S. Gov't

Figure

• 

  Fig. 1 Molecular analysis of HLA-DRB/HLA-DQB genes in a case of ADEM with the western genotype of multiple sclerosis. PCR-SSOP (A), PCR-SSP (B) with gel Immunoelectrophoresis for HLA-DQB (C) illustrates HLA-DRB1*1501 and HLA-D QB1*0602/0604.

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